Device

Part:BBa_K561000:Design

Designed by: HUA Yijie, PI Ruoxi, XIA Yu   Group: iGEM11_ZJU-China   (2011-09-29)


Pvgb+YFP+tetR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Because vgb promoter induces high expression under microaerobic conditions and induces lower expression under anaerobic conditions, we used medium RBS to resist it from inducing expression under anaerobic conditions.


Source

vgb promoter comes from Vitreoscilla sp.

References